About OligoFaktory Standalone Edition
The user interface emphasize useability and is aimed at assisting researchers for a painless, rapid, automated, and reliable design. A master-detail interface allow users to enter their sequences and related regions. Users can cut-and-paste query DNA into the Editor view, or they can use the File > Import? menu item to import FASTA files. Several design iterations can be applied, using five complementary actions:
- The Design Oligos action allows for the automated design of specific long oligonucleotides on an arbitrary number of ORFs (e.g., for the development of DNA microarrays).
- The Design Primers action allows for the automated design of specific PCR primer pairs on an arbitrary number of regions.
- The Design SiRNAs action allows designing optimal 19bp siRNA with the method described in Rational siRNA design for RNA interference, A. Reynolds et al. Nature biotechnology, 2004, vol 22, 326-330.
- The Detect Repetitions action identifies all repetitions and microsatelites under user-specified constraints.
- Many more features.
